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KMID : 0545120070170061041
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Journal of Microbiology and Biotechnology
2007 Volume.17 No. 6 p.1041 ~ p.1044
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Genetic Variability and Phylogenetic Relationship Among Proton-Beam-Irradiated Strains of Pleurotus ostreatus
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Kwon Hye-Jin
Park Yong-Jin
Yoo Young-Bok
Park Soon-Young
Kong Won-Sik
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Abstract
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To assess the effects of a proton beam on oyster mushrooms (Pleurotus ostreatus), the genetic diversity and phylogenetic relationships among strains induced by a proton beam were investigated based on a clustering analysis. According to an AFLP DNA polymorphism analysis, the induced strains were divided into four groups that coincided with the dose. When applying proton-beam radiation, the dissimilarity among the induced strains increased when increasing the dose. When using more than 400 Gy, the genetic dissimilarity of the irradiated strains was 46-58%. Thus, evaluating the induced strains using the AFLP technique was effective in revealing the mutation effect of the proton beam.
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KEYWORD
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AFLPs, mutation, oyster mushroom (Pleurotus ostreatus), polymorphism, proton beam
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